Courses

From SC2.0

Contents 1 Intersession 2008 2 Fall 2007 3 Protocols 4 Links 5 Archived course materials

[edit] Intersession 2008

• Chunk/BB Assignments - BAG2008IS
• Course Materials Plate layouts, sequences, etc.
• Calendar

[edit] Fall 2007

• Recommended reading list: There is no textbook assigned for this course. We do, however, expect you to have a complete understanding of the underlying technical principles and background theory: not only will this help you work more efficiently, you will get a lot more out of the course if you know the details of what you are actually doing. Please be familiar with this material prior to the beginning of the course. If necessary, we will review basic theory in review sessions.

• Groups - BAG2007f

• Lab Meeting Room Assignments

• Calendar

• Assignments - BAG2007f

• 3L Oligo Plate Layouts

[edit] Protocols

• Nomenclature

• Oligos

• Positive Controls

• Templateless PCR

• Finish PCR

• Ligation and Transformation

• Colony Screening PCR

• Gel Electrophoresis

• 9600 Programs

• Recipes

• Start to Finish protocols DO NOT USE THESE AT THE MOMENT! WAIT FOR ME TO UPDATE THEM ACCORDING TO OUR NEW PRIMER CONCENTRATIONS.

[edit] Links

• GeneDesign

• SGD

Tutorials

• Sequencing animation

• Gel electrophoresis animation

• Restriction digest animation

• Transformation overview animation

• Transformation - the nitty gritty - animation

• PCR animation

• Biological Assays A great place to start reading about dilutions and the Spec 20.

Sequence Analysis Tools

• FinchTV Sequence viewer for PCs and Macs

• ClustalW Alignment program

[edit] Archived course materials

• Fall 2007
• Summer 2007
• Old Protocols